Identification of Subunits of Mb304 Transcription Factor Regulating Cell Division Cycle in Saccharomyces cerevisiae

Abstract

Cell division is the most important phenomenon for reproduction and the continuity of the life. Each of the stages of the cell division (G1, G2, M, S) affects all the process and the importance of this process’s control is of vital importance. Checkpoints regulated by proteins and different transcription factors are responsible for this process. The identification of any regulatory factor of the cell divison cycle is of great significance for achieving a deeper understand of the cell cycle, errors in the cell cycle (cancer mechanism) and complex processes, such as aging, senescence etc. Our research group aimed the identification of the subunits of Mb304 which are involved in the cell growth/size regulation. Previous studies have shown that two of the subunits, reciprocally Mb304-a and Mb304-b of Mb304 complex are directly involved in the regulation of the checkpoint G1/S and in the editing of DNA errors.

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Identification of Subunits of Mb304 Transcription Factor Regulating Cell Division Cycle in Saccharomyces cerevisiae 

Cell division is the most important phenomenon for reproduction and the continuity of the life. Each of the stages of the cell division (G1, G2, M, S) affects all the process and the importance of this process’s control is of vital importance.

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Photosynthesis and Quantum Biology

The electron excitation in the antenna (which varies between organisms) of the photosynthetic organisms enables the light absorption. The antenna of the bacteria can be in the form of the ring, while the chlorophyll pigments acts as antenna in the plants and higher organisms and its function is the absorption of the photons[1]. Continue reading “Photosynthesis and Quantum Biology”

Compare and contrast eukaryotic and prokaryotic mRNAs and mode of translation initiation

Prokaryotic mRNA is most of the times polycistronic, while the eukaryotic mRNA is monocistronic. The 5′-7 methyl guanosine is absent in prokaryotes, while in eukaryotes is present.

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Enzymatic Acivity

   Table 1. Enzyme assay reaction were prepared according below table. After adding 0,1%SDS mixture were vortex at 28℃ for 5 minutes. Incubation of tubes occurs at 28℃ for 15 minutes. After adding 1M Na2COmixture was vortex. After all reaction mixture were centrifuged and debris was discarded. Remaining part taken as 200 μl was added into well plate from F1 to F7 with respectively. Spectrophotometric measurements were applied at 420 nm. 

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Enzymes

Table 1. Substrate preparation were done by applying indicated amounts of ONPG by simple dilution. Stock solution of ONPG was 15mg/ml.Then, reaction mixture were prepared by adding Z buffer, Chloroform, 0.1 % SDS by respective manner. Na2COwas added to end the reaction.Each tube contained 800 μl cell lysate.  

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Glycogen

Benedict Test is method detect carbohydrate by using reducing properties of them. Some of the carbohydrates can reduce metals, in Benedict Test this metal is Copper, so color change could be observed.In reaction Copper +2 reduce to Copper+1 and resulting color change.

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